Cormoran® Digital 4x dPCR Master Mix

Cormoran® Digital 4× dPCR Master Mix is a competitively priced hot-start master mix for probe-based digital PCR, tested on QIAcuity®.

Aptamer-controlled hot start

The aptamer-controlled hot-start chemistry limits non-specific primer extension during reaction setup. A reference dye for partition identification is already included in the supplied formulation.

Application data: comparison with the QIAcuity® Probe PCR Kit

The accompanying benchmark figure shows a direct comparison in a BRAF WT/V600E duplex development experiment performed on QIAcuity®.

The Cormoran formulation produced distinct positive and negative fluorescence populations. In the illustrated experiment, V600E was detected at an input of 100 mutant copies in a background of 49,000 wild-type copies per reaction.

These example development data were generated by Hahn-Schickard using synthetic gBlock DNA and modular reporter chemistry.

Digital PCR assay kits in development

We are also developing target-specific dPCR assay kits in collaboration with Hahn-Schickard, based on their modular reporter chemistry. Cormoran Biotech holds a licence to this reporter technology. The first kits are expected to become available soon.

Custom formulations and bulk supply

Assay-specific formulation adjustments and bulk quantities are available on request.

Key features

• 4× concentrated formulation for probe-based digital PCR research.

• For target-specific DNA assay development and reagent evaluation.

• Direct access to scientific support and formulation expertise.

• Custom formulations and bulk supply on request.